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2 dic 2020Invisible Women
Invisible Women is a social project aimed at raising awareness about the importance of hygiene during the menstrual period of homeless women in the city of Campinas-São Paulo-Brazil. In the absence of adequate hygiene conditions, especially during menstruation, they use old rags and leftover food to stop menstruation. In view of this scenario, it is intended to provide sanitary pads free of charge to underprivileged and homeless women via a health or social assistance agency. After a period of observation in the central area of the city, it was noticed the lack of access of women to the appropriate means of hygiene, and harms their health, since they have no viable options for staying clean. At the same time, an internet search directed to the assistance segments of the municipality, such as the Archdiocese of Campinas, established contact with those responsible for the Consultório na Rua, which gives attention to the health of street women. Observation scripts and questions to be applied in the interviews with the Consultório na Rua team and the homeless people were elaborated. Such interviews were conducted with a team of professionals and with a homeless person, and recorded on audio, after the interviewees signed free consent terms. The audios of the interviews were transcribed and used in the elaboration and development of actions. Partially, it was possible to recognize that homeless women are subject to physical, emotional and psychological vulnerability, using harmful means of survival, with poor hygiene being one of them.
1 dic 2020Pinion shell (Araucaria Angustifolia) as a reducer and stabilizer agent to obtain silver nanoparticles in a sustainable way
This work aims to develop a green method for Silver Nanoparticles (NPsAg) synthesis using raw pinion shell extract. Metallic nanoparticles, including silver ones, are particles that have diverse properties, such as high proportion between surface area and volume and higher reactivity. The NPsAg stand out for exhibiting important properties, such as antimicrobial activity, and a large application field, like in water treatment, among others. The methods of nanoparticle synthesis usually employ agressives reducing and stabilizing agents, such as sodium borohydride and hydrazine. As a result, many studies of green synthesis have been developed using plant extracts rich in phenolic compounds and ascorbic acid, which promote the reduction and stabilization of nanoparticles. These researches corroborate with our proposal, which aims to use pinion shell extract, rich in phenolic compounds, for the synthesis of NPsAg. The pinion shell compounds were extracted using non-toxic solvents such as water and ethanol in different concentrations, temperatures and pH values. After, qualitative tests of phenolic compounds were performed and one of the extracts was used to obtain the nanoparticles using silver nitrate at pH eight as the precursor salt. Characterization tests will still be performed to prove the formation and stabilization of silver nanoparticles.
- 30 nov 2020
Chromosome organisation during cell division and effects on nuclear shape in interphase
Abnormally shaped, lobed nuclei are observed in cancer cells, although the mechanisms causing the misshapen are unknown. Here, the effects are measured as solidity (roundness). One result of abnormal nuclear shape is that misshapen cell nuclei may alter gene expression dramatically shifting normal cell activity and contributing to diseases. Many proteins including the class of molecular motors called kinesins Kif18A direct regular cell activities including cell division. The Kif18A is responsible for maintaining chromosome alignment in the middle of a dividing cell before chromosomes segregate,producing two daughter cells. Without Kif18A chromosomes are unaligned at metaphase causing disordered chromosome segregation. In this study,it was investigated whether proper chromosome alignment during cell division impacts the resulting nuclear shape of daughter cells. Live cells transfected with a fluorescently labelled plasmid (GFP-H2B to label chromatin) were imaged and analysed using program Image J to observe chromosome dynamics and cell shape after division. In the absence of Kif18A, daughter cell nuclei had lower solidity measurements, meaning nuclei were more abnormally shaped (p being less than 0.0001). Furthermore, in the absence of Kif18A, cells were more likely to have lagging chromosomes which led to the production of micronuclei (whole or fragmented chromosomes separated from the main nucleus). Additionally, population wide Kif18A mutants had more cells with abnormally shaped nuclei compared to the control. These results suggest that chromosome alignment during cell division impacts nuclear shape of daughter cells, and is a possible mechanism leading to abnormal nuclear shape.
30 nov 2020Chromosome organisation during cell division and effects on nuclear shape in interphase
Abnormally shaped, lobed nuclei are observed in cancer cells, although the mechanisms causing the misshapen are unknown. Here the effects of chromosome organisation during cell division on nuclear shape were measured as solidity (roundness). One consequence of abnormal nuclear shape is that misshapen cell nuclei may alter gene expression dramatically shifting normal cell activity and contributing to diseases. Many proteins including the class of molecular motors called kinesins, Kif18A direct regular cell activities including cell division. The Kif18A is responsible for maintaining chromosome alignment in the middle of a dividing cell before chromosomes segregate, producing two daughter cells. Without Kif18A, chromosomes are unaligned at metaphase, causing disordered chromosome segregation. In this study, it was investigated whether proper chromosome alignment during cell division impacts the resulting nuclear shape of daughter cells. Live cells transfected with a fluorescently labelled plasmid (GFP-H2B,to label chromatin)were imaged and analysed using program Image J to observe chromosome dynamics and cell shape after division. In the absence of Kif18A, daughter cell nuclei had lower solidity measurements, meaning nuclei were more abnormally shaped (p is less than 0.0001). Furthermore, in the absence of Kif18A, cells were more likely to have lagging chromosomes which led to the production of micronuclei (whole or fragmented chromosomes separated from the main nucleus). Additionally, population wide, Kif18A mutants had more cells with abnormally shaped nuclei compared to the control. These results suggest that chromosome alignment during cell division impacts nuclear shape of daughter cells,and is a possible mechanism leading to abnormal nuclear shape.
26 nov 2020Pine Shell (Araucaria Angustifolia) as a reducer and stabilizer agent to obtain silver nanoparticles in a sustainable way
This work aims to develop a green method for Silver Nanoparticles (NPsAg) synthesis using raw pinion shell extract. Metallic nanoparticles, including silver ones, are particles that have diverse properties, such as high proportion between surface area and volume and higher reactivity. The NPsAg stand out for exhibiting important properties, such as antimicrobial activity, and a large application field, like in water treatment, among others. The methods of nanoparticle synthesis usually employ agressives reducing and stabilizing agents, such as sodium borohydride and hydrazine. As a result, many studies of green synthesis have been developed using plant extracts rich in phenolic compounds and ascorbic acid, which promote the reduction and stabilization of nanoparticles. These researches corroborate with our proposal, which aims to use pinion shell extract, rich in phenolic compounds, for the synthesis of NPsAg. The pinion shell compounds were extracted using non-toxic solvents such as water and ethanol in different concentrations, temperatures and pH values. After, qualitative tests of phenolic compounds were performed and one of the extracts was used to obtain the nanoparticles using silver nitrate at pH eight as the precursor salt. Characterization tests will still be performed to prove the formation and stabilization of silver nanoparticles.
- 26 nov 2020
Chromosome organisation during cell division and effects on nuclear shape in interphase
Abnormally shaped,lobed nuclei are observed in cancer cells, although the mechanisms causing the misshapen are unknown. Here, the effects of chromosome organisation during cell division on nuclear shape were measured as solidity. One result of abnormal nuclear shape is that misshapen cell nuclei may alter gene expression, dramatically shifting normal cell activity and contributing to diseases. Many proteins including the class of molecular motors, called kinesins is responsible for maintaining chromosome alignment in the middle of a dividing cell before chromosomes segregate, producing two daughter cells. Without Kif18A, chromosome are unaligned at metaphase, causing disordered chromosome segregation. In this study,it was investigated whether proper chromosome alignment during cell division impacts the resulting nuclear shape of daughter cells. Live cells transfected with a fluorescently labelled plasmid (GFP-H2B,to label chromatin) were imaged and analysed using the program Image J to observe chromosome dynamics and cell shape after division. In the absence of Kif18A, daughter cell nuclei had lower solidity measurements, meaning nuclei were more abnormally shaped (p is less than 0.0001). Furthermore, in the absence of Kif18A, cells were more likely to have lagging chromosomes which led to the production of micronuclei (whole fragmented chromosomes separated from the main nucleus). Additionally, population wide, Kif18A mutants had more cells with abnormally shaped nuclei compared to the control. These results suggest that chromosome alignment during cell division impacts the resulting nuclear shape of daughter cells, and is a possible mechanism leading to abnormal nuclear shape.
26 nov 2020Chromosome organisation during cell division and effects on nuclear shape in interphase
Abnormally shaped,lobed nucleic are observed in cancer cells, although the mechanisms causing the misshapen are unknown. Here the effects are measured as solidity (roundness). One consequence of abnormal nuclear shape is that misshapen cell nuclei may alter gene expression, dramatically shifting normal cell activity and contributing to diseases. Many proteins, including the class of molecular motors,called kinesins,direct regular cell activities including cell division. The Kif18A is responsible for maintaining chromosome alignment in the middle of a dividing cell before chromosome segregate, producing two daughter cells. Without Kif18A, chromosomes are unaligned at metaphase, causing disordered chromosome segregation. In this study,it was investigated whether chromosome alignment during cell division impacts the resulting nuclear shape of daughter cells. Live cells were transfected with a fluorescently labelled plasmid (GFP-H2B),to label chromatin were imaged and analysed using the program Image J to observe chromosome dynamics and cell shape after division. In the absence of Kif18A, daughter cell nuclei had lower solidity measurements, meaning nuclei were more abnormally shaped (p less than 0.0001). Furthermore, in the absence of Kif18A, cells were more likely to have lagging chromosomes which led to the production of micronuclei (whole or fragmented chromosomes separated from the main nucleus). Additionally, population wide, Kif18A mutants had more cells with abnormally shaped nuclei compared to the control. These results suggest that chromosome alignment during cell division impacts nuclear shape of daughter cells,and is a possible mechanism leading to abnormal nuclear shape.
26 nov 2020Pine Shell (Araucaria Angustifolia) as a reducer and stabilizer agent to obtain silver nanoparticles in a sustainable way
This work aims to develop a green method for Silver Nanoparticles (NPsAg) synthesis using raw pinion shell extract. Metallic nanoparticles, including silver ones, are particles that have diverse properties, such as high proportion between surface area and volume and higher reactivity. The NPsAg stand out for exhibiting important properties, such as antimicrobial activity, and a large application field, like in water treatment, among others. The methods of nanoparticle synthesis usually employ agressives reducing and stabilizing agents, such as sodium borohydride and hydrazine. As a result, many studies of green synthesis have been developed using plant extracts rich in phenolic compounds and ascorbic acid, which promote the reduction and stabilization of nanoparticles. These researches corroborate with our proposal, which aims to use pinion shell extract, rich in phenolic compounds, for the synthesis of NPsAg. The pinion shell compounds were extracted using non-toxic solvents such as water and ethanol in different concentrations, temperatures and pH values. After, qualitative tests of phenolic compounds were performed and one of the extracts was used to obtain the nanoparticles using silver nitrate at pH eight as the precursor salt. Characterization tests will still be performed to prove the formation and stabilization of silver nanoparticles.
7 nov 2020Electricity from pure Ash
The way Ash waste is disposed is causing some effects when the ash gets into contact with the air resulting in air pollution. This polluted air is causing some health effects to people leading to pneumatic diseases and other related lung diseases. As a way to manage this issue , the produced ash from burning firewood can be used to generate electricity for use in our homes.
- 7 nov 2020
Electricity from Ash
Wrong disposal of Ash waste is causing a lot of air pollution that is affecting people's health as they inhale the polluted air causing diseases such as lung cancer and other pneumatic diseases. As a way Ash can be used to produce electricity that can be used in homes.